RDA Instrument Processing, Sterilization, and Monitoring 2

Question 1

What is the correct temperature and time for a gravity displacement steam autoclave to achieve sterilization of unwrapped instruments?

Accepted Answer

250°F (121°C) for 15–30 minutes

Answer

Gravity displacement steam autoclaves sterilize at 250°F (121°C) for 15–30 minutes (depending on load type). Pre-vacuum autoclaves operate at 270°F (132°C) for shorter cycles.

Question 2

Which biological indicator is used to verify steam autoclave sterilization efficacy?

Accepted Answer

Geobacillus stearothermophilus spore strips

Answer

Geobacillus stearothermophilus (formerly Bacillus stearothermophilus) spore strips are the biological indicator for steam autoclave validation, as these spores are highly resistant to moist heat.

Question 3

What is the purpose of a chemical indicator (CI) placed inside instrument packaging before sterilization?

Accepted Answer

To indicate that the package was exposed to sterilization conditions (heat, pressure, or chemical)

Answer

Chemical indicators (Type 1–6) change color or form when exposed to sterilization conditions, indicating that the package was processed. They do NOT confirm that sterilization was achieved.

Question 4

Which classification of instruments requires sterilization because they penetrate soft tissue or bone?

Accepted Answer

Critical instruments

Answer

Critical instruments (e.g., forceps, elevators, scalers, burs, needles) penetrate soft tissue or bone and must be sterilized (not just disinfected) because they have the highest risk for transmitting infection.

Question 5

What is the correct first step in instrument reprocessing after use?

Accepted Answer

Transport to the sterilization area in a covered container

Answer

The first step is safely transporting used instruments in a covered, puncture-resistant container to the sterilization area to prevent occupational exposure during transport.

(RDA) Registered Dental Assistant Practice Test

(RDA) Registered Dental Assistant Practice Test

RDA Instrument Processing, Sterilization, and Monitoring 2