Cord Blood Cord Blood Stem Cells 2

Question 1

What is the minimum total nucleated cell (TNC) dose per kilogram of recipient body weight typically required for successful cord blood engraftment?

Accepted Answer

2.5 × 10⁷ TNC/kg

Answer

A minimum of approximately 2.5 × 10⁷ total nucleated cells per kilogram of recipient body weight is generally required to achieve reliable engraftment.

Question 2

What is mixed chimerism following a cord blood transplant?

Accepted Answer

A mixture of both donor and recipient blood cells coexisting

Answer

Mixed chimerism is a state where both donor-derived and recipient-derived blood cells coexist in the recipient after transplantation.

Question 3

How long can cord blood stem cells be stored cryogenically while maintaining viability?

Accepted Answer

25 years or more

Answer

Studies have confirmed that cord blood stem cells stored in liquid nitrogen at −196°C can remain viable for 25 years or more.

Question 4

What is ex vivo expansion of cord blood stem cells designed to overcome?

Accepted Answer

The limited cell dose in a single cord blood unit

Answer

Ex vivo expansion protocols aim to increase the total cell dose from a single cord blood unit, which is often insufficient for adult patients.

Question 5

Which cytokine combination is commonly used in ex vivo cord blood stem cell expansion protocols?

Accepted Answer

SCF, TPO, and Flt3 ligand

Answer

Stem cell factor (SCF), thrombopoietin (TPO), and Flt3 ligand are key cytokines used to promote ex vivo expansion of cord blood hematopoietic progenitors.

Cord Blood Practice Test

Cord Blood Practice Test

Cord Blood Cord Blood Stem Cells 2