Cord Blood Cord Blood Collection and Processing

Question 1

When is cord blood collected relative to the birth process?

Accepted Answer

Before delivery of the placenta, immediately after the umbilical cord is clamped and cut

Answer

Several hours before delivery begins

Answer

Only after a cesarean section

Answer

After the mother has been discharged from the hospital

Question 2

What is the average volume of cord blood collected from a single birth?

Accepted Answer

60–80 mL

Answer

500–700 mL

Answer

100–200 mL

Answer

10–20 mL

Question 3

What anticoagulant is pre-loaded in cord blood collection bags to prevent clotting?

Accepted Answer

Citrate phosphate dextrose (CPD)

Answer

Warfarin

Answer

Heparin sulfate

Answer

Aspirin solution

Question 4

Within how many hours after collection must cord blood be processed to maintain optimal cell viability?

Accepted Answer

Within 48 hours

Answer

Within 6 hours

Answer

Within 12 hours

Answer

Within 7 days

Question 5

What is the primary purpose of the density gradient centrifugation step in cord blood processing?

Accepted Answer

To separate mononuclear cells (including stem cells) from red blood cells and granulocytes

Answer

To freeze the cord blood

Answer

To add DMSO to the sample

Answer

To test for bacterial contamination

Question 6

What is the minimum total nucleated cell (TNC) count generally required for a cord blood unit to qualify for public banking?

Accepted Answer

5 × 10⁸ cells

Answer

5 × 10⁵ cells

Answer

1 × 10⁶ cells

Answer

2 × 10⁷ cells