VTNE Laboratory Procedures 2

Question 1

Which anticoagulant is found in a purple-top (lavender) tube and is used for complete blood counts?

Accepted Answer

EDTA

Answer

EDTA (ethylenediaminetetraacetic acid) chelates calcium and is the anticoagulant of choice for CBCs because it preserves cell morphology.

Question 2

A urine specific gravity of 1.008–1.012 in a dog is classified as:

Accepted Answer

Isosthenuria

Answer

Isosthenuria refers to urine specific gravity in the range of 1.008–1.012, which is similar to glomerular filtrate and indicates inadequate concentrating or diluting ability.

Question 3

When performing a blood smear, the feathered edge is used to evaluate:

Accepted Answer

Platelet clumps and large cells

Answer

The feathered edge of a blood smear is where platelets tend to clump and large cells such as monocytes accumulate, making it an important area to scan.

Question 4

Which stain is used to identify reticulocytes in a peripheral blood smear?

Accepted Answer

New methylene blue stain

Answer

New methylene blue is a supravital stain that precipitates residual RNA in reticulocytes, making them identifiable as cells with blue granular network.

Question 5

A sample for blood glucose measurement is collected in a gray-top tube. What additive does it contain?

Accepted Answer

Sodium fluoride and potassium oxalate

Answer

Gray-top tubes contain sodium fluoride (a glycolytic inhibitor) and potassium oxalate (an anticoagulant), preserving glucose levels for accurate measurement.

VTNE - Veterinary Technician National Examination Practice Test

VTNE - Veterinary Technician National Examination Practice Test

VTNE Laboratory Procedures 2