Cord Blood Cord Blood Collection and Processing

Question 1

When is cord blood collected relative to the birth process?

Accepted Answer

Before delivery of the placenta, immediately after the umbilical cord is clamped and cut

Answer

Cord blood is collected immediately after the umbilical cord is clamped and cut following birth, and before or after delivery of the placenta, using a sterile needle to drain blood from the umbilical vein.

Question 2

What is the average volume of cord blood collected from a single birth?

Accepted Answer

60–80 mL

Answer

The average cord blood collection volume is approximately 60–80 mL, though this varies based on birth weight, timing of cord clamping, and collection technique.

Question 3

What anticoagulant is pre-loaded in cord blood collection bags to prevent clotting?

Accepted Answer

Citrate phosphate dextrose (CPD)

Answer

Cord blood collection bags are pre-filled with citrate phosphate dextrose (CPD), which chelates calcium to prevent coagulation and preserve cell viability.

Question 4

Within how many hours after collection must cord blood be processed to maintain optimal cell viability?

Accepted Answer

Within 48 hours

Answer

Cord blood should ideally be processed within 48 hours of collection to maintain acceptable cell viability, though processing within 24 hours is preferred by most accreditation standards.

Question 5

What is the primary purpose of the density gradient centrifugation step in cord blood processing?

Accepted Answer

To separate mononuclear cells (including stem cells) from red blood cells and granulocytes

Answer

Density gradient centrifugation uses a ficoll or similar medium to separate the buoyant mononuclear cell layer (containing stem cells) from denser red blood cells and granulocytes.

Cord Blood Practice Test

Cord Blood Practice Test

Cord Blood Cord Blood Collection and Processing