FREE CT Cytopreparation Techniques Questions and Answers

Question 1

Which fixative is most commonly used for preparing Pap smears?

Accepted Answer

95% Ethanol

Answer

95% Ethanol is the most commonly used and recommended fixative for preparing Pap smears. Its primary function is to rapidly penetrate and preserve cellular morphology by coagulating proteins, preventing air-drying artifacts, and maintaining nuclear and cytoplasmic detail. Proper fixation is crucial for accurate cytological interpretation and diagnostic reliability.

Question 2

What is the advantage of liquid-based cytology over conventional smear techniques?

Accepted Answer

Cleaner background with better cell preservation

Answer

The main advantage of liquid-based cytology (LBC) over conventional smear techniques is its ability to produce slides with a cleaner background and superior cell preservation. LBC methods remove obscuring elements like blood, mucus, and inflammation, and evenly distribute cells in a monolayer. This significantly improves visualization of cellular details, reduces artifacts, and enhances diagnostic accuracy.

Question 3

Which stain is typically used for non-gynecological cytology specimens like fine needle aspirations (FNAs)?

Accepted Answer

Diff-Quik

Answer

Diff-Quik is a rapid stain commonly used for FNAs and other non-gynecological cytology samples.

Question 4

Why is rapid on-site evaluation (ROSE) performed during fine needle aspirations (FNA)?

Accepted Answer

To confirm specimen adequacy on-site

Answer

ROSE allows immediate assessment of sample adequacy, reducing repeat procedures.

Question 5

Which of the following is a cytocentrifugation technique primarily used for?

Accepted Answer

Concentrating cells onto a slide

Answer

Cytocentrifugation concentrates cellular material onto a small slide area for improved microscopic examination.

CT - Certified Cytotechnologist Practice Test

CT - Certified Cytotechnologist Practice Test

FREE CT Cytopreparation Techniques Questions and Answers