The Comet assay, also known as single-cell gel electrophoresis, is used to detect DNA strand breaks in individual cells by visualizing DNA migration patterns.
The LD50 test is used to determine the dose of a substance that is lethal to 50% of the test population, providing a measure of the substance's acute toxicity.
In vitro methods offer a faster, more controlled environment for testing and reduce ethical concerns associated with animal testing.
The Ames test is an in vitro assay used to assess the mutagenic potential of chemical compounds by observing their ability to induce mutations in the DNA of bacteria.
The MTT assay is a colorimetric assay for assessing cell metabolic activity, commonly used to measure cytotoxicity by determining the viability of cells after exposure to chemicals.