ABT Cheat Sheet 2026

The 30 highest-yield ABT facts, distilled from real exam questions. Print it, save it as a PDF, or study it here — free, no sign-up.

1. Which test in the developmental toxicity battery specifically evaluates learning and memory deficits caused by in utero chemical exposure? → Developmental neurotoxicity (DNT) study
2. Which vitamin, when consumed in excessive amounts, can lead to toxicity known as hypervitaminosis? → Vitamin A
3. What is the primary concern of nutritional toxicology? → Studying the adverse effects of chemicals in food on human health
4. Which male reproductive toxicant causes depletion of spermatogonia by alkylating DNA in the seminiferous epithelium? → Dibromochloropropane (DBCP)
5. Which antidote is used for organophosphate poisoning to reactivate acetylcholinesterase? → Pralidoxime (2-PAM)
6. Methylmercury exposure in humans primarily occurs through: → Consumption of contaminated fish and seafood
7. DEHP (di-2-ethylhexyl phthalate) exerts reproductive toxicity in male rodents primarily by disrupting: → Testosterone biosynthesis via Leydig cell PPAR-α activation affecting steroidogenesis
8. Aflatoxins, which can contaminate food supplies, are produced by which type of organism? → Fungi
9. Which type of toxicant is most likely to cause damage to the respiratory system? → Pulmonotoxins
10. Which naturally occurring toxin found in certain legumes can cause hemolytic anemia if consumed in large amounts? → Phytohemagglutinin
11. ICH S5(R3) guidelines govern which aspect of pharmaceutical development? → Detection of reproductive and developmental toxicity for human pharmaceuticals
12. Which specific antidote reverses benzodiazepine-induced CNS and respiratory depression? → Flumazenil
13. What does the Ames test evaluate? → Mutagenic potential
14. Chain of custody in forensic toxicology is essential primarily to: → Document sample handling to preserve legal admissibility of results
15. Which specimen is most useful for detecting drug exposure in neonates born to substance-using mothers? → Meconium
16. Physostigmine is the preferred antidote for severe toxicity from which drug class? → Anticholinergics
17. Which toxin causes a characteristic 'garlic breath' odor and is associated with Mees' lines on fingernails? → Arsenic
18. Which anti-acne drug is required to be dispensed through the iPLEDGE REMS program because of severe teratogenicity? → Isotretinoin (Accutane)
19. Carbon monoxide poisoning is treated with 100% oxygen primarily because it: → Competitively displaces CO from hemoglobin by mass action
20. The EPA's Reference Dose (RfD) is defined as an estimate of daily exposure that: → Is likely to be without appreciable risk of deleterious effects over a lifetime
21. Chronic beryllium disease (CBD/berylliosis) is best characterized as: → A granulomatous lung disease from cell-mediated hypersensitivity to beryllium
22. Which testing guideline is commonly used internationally for assessing the skin irritation potential of chemicals? → OECD TG 404
23. Which mechanism explains salicylate-induced hyperthermia in severe toxicity? → Uncoupling of oxidative phosphorylation
24. Occupational asthma caused by toluene diisocyanate (TDI) primarily involves which immunological mechanism? → IgE-mediated (Type I) hypersensitivity
25. Which phase II biotransformation reaction is most important for detoxifying reactive electrophilic metabolites via conjugation with a tripeptide? → Glutathione conjugation (GST)
26. Which cytochrome P450 enzyme is responsible for metabolizing the largest number of pharmaceutical drugs (approximately 30–40%)? → CYP3A4
27. Which of the following in vitro methods is commonly used to assess cytotoxicity of chemicals? → MTT assay
28. The presence of which compound in improperly stored potatoes can cause gastrointestinal and neurological disorders? → Solanine
29. Fentanyl analogs such as carfentanil are classified as Schedule I substances and are most commonly detected in forensic cases involving: → Opioid overdose deaths with no prescribed opioid use
30. In workplace drug testing regulated by SAMHSA/DOT, which five drug classes must be tested in the federal 5-panel screen? → Amphetamines, cocaine, opioids, PCP, marijuana (THC)