CCTC Organ Procurement and Preservation 2

Question 1

Which component of University of Wisconsin (UW) solution primarily prevents cellular swelling during cold storage?

Accepted Answer

Lactobionate

Answer

Lactobionate is an impermeant anion in UW solution that maintains osmotic balance outside the cell, preventing cellular swelling during hypothermic storage.

Question 2

What is the primary mechanism of organ injury during reperfusion following cold ischemia?

Accepted Answer

Generation of reactive oxygen species causing oxidative damage

Answer

Ischemia-reperfusion injury occurs when reintroduction of oxygen leads to a burst of reactive oxygen species that damage cellular membranes and trigger an inflammatory cascade.

Question 3

Machine perfusion of kidneys offers which primary advantage over static cold storage?

Accepted Answer

Allows continuous viability assessment and active removal of ischemic byproducts

Answer

Machine perfusion maintains metabolic activity, continuously flushes harmful metabolic byproducts, and enables real-time assessment of renal resistance and perfusate biomarkers.

Question 4

What is the generally accepted maximum cold ischemia time for a cardiac transplant?

Accepted Answer

4–6 hours

Answer

Heart transplants require cold ischemia times under 4–6 hours because the heart is exceptionally sensitive to prolonged ischemic injury.

Question 5

Compared to DBD (donation after brain death) organs, DCD organs have a higher risk of which complication?

Accepted Answer

Delayed graft function

Answer

DCD kidneys experience warm ischemia during the withdrawal of life support and cardiac arrest period, leading to tubular injury and increased rates of delayed graft function.

CCTC - Certified Clinical Transplant Coordinator Practice Test

CCTC - Certified Clinical Transplant Coordinator Practice Test

CCTC Organ Procurement and Preservation 2