ALAT ALAT Research Procedures and Techniques Questions and Answers 3

Question 1

Which needle gauge is most appropriate for intravenous injection in a mouse tail vein?

Accepted Answer

25–27 gauge

Answer

A 25–27 gauge needle is appropriate for mouse tail vein IV injections due to the small size of the vessel.

Question 2

What is the term for the dose of a substance given per unit of body weight in animal research?

Accepted Answer

mg/kg

Answer

Doses in animal research are expressed as mg/kg (milligrams per kilogram of body weight) to normalize for animal size.

Question 3

Before administering any experimental compound to a laboratory animal, the ALAT should verify:

Accepted Answer

That the compound has been approved by the IACUC protocol

Answer

All experimental procedures, including compound administration, must be approved and covered under the IACUC protocol before they can be performed.

Question 4

Which of the following is the correct site for subcutaneous injection in a mouse?

Accepted Answer

Scruff of the neck

Answer

The scruff of the neck (loose skin over the dorsal neck/shoulder) is the standard site for subcutaneous injections in mice.

Question 5

What does 'PO' mean as a route of administration in laboratory animal research?

Accepted Answer

Per os (oral administration)

Answer

PO stands for 'per os,' meaning the substance is administered orally, either by voluntary ingestion or gavage.

ALAT - Assistant Laboratory Animal Technician Practice Test