ALAT ALAT Research Procedures and Techniques Questions and Answers 1 — Questions and Answers
Question 1: Which route of administration is used when injecting a substance directly into the abdominal cavity of a rodent?
- Intravenous (IV)
- Subcutaneous (SC)
- Intraperitoneal (IP) (Correct answer)
- Intramuscular (IM)
Correct answer: Intraperitoneal (IP)
Intraperitoneal (IP) injection delivers substances directly into the abdominal cavity and is a common route in rodent research.
Question 2: What is the maximum recommended volume for a single subcutaneous injection in a mouse?
- 0.1 mL
- 0.5 mL (Correct answer)
- 2.0 mL
- 5.0 mL
Correct answer: 0.5 mL
The maximum recommended subcutaneous injection volume for a mouse is 0.5 mL to avoid tissue damage.
Question 3: When performing a blood collection from the tail vein of a rat, which technique is commonly used to dilate the vessel?
- Applying ice to the tail
- Warming the tail in warm water (Correct answer)
- Applying a tourniquet above the collection site
- Shaving the tail fur
Correct answer: Warming the tail in warm water
Warming the tail in warm water causes vasodilation, making the tail vein easier to access for blood collection.
Question 4: What is the purpose of a gavage needle used in laboratory animal procedures?
- To collect blood samples from the jugular vein
- To deliver substances directly into the stomach via the esophagus (Correct answer)
- To administer intramuscular injections
- To perform ear notch identification
Correct answer: To deliver substances directly into the stomach via the esophagus
A gavage needle is a blunt-tipped curved needle used to deliver compounds directly into the stomach via oral gavage.
Question 5: Which of the following is a proper technique when performing intramuscular injections in rabbits?
- Injecting into the scruff of the neck
- Injecting into the epaxial muscles of the lumbar region (Correct answer)
- Injecting into the tail base
- Injecting into the footpad
Correct answer: Injecting into the epaxial muscles of the lumbar region
The epaxial (lumbar) muscles are the preferred IM injection site in rabbits due to their size and accessibility.
Question 6: What does the term 'aseptic technique' mean in the context of laboratory animal procedures?
- Using anesthesia before any procedure
- Preventing contamination of sterile areas with microorganisms (Correct answer)
- Disposing of sharps in a red biohazard bag
- Recording all procedures in the animal's health record
Correct answer: Preventing contamination of sterile areas with microorganisms
Aseptic technique refers to practices that prevent microbial contamination during surgical or invasive procedures.
ALAT ALAT Research Procedures and Techniques Questions and Answers 1
Which route of administration is used when injecting a substance directly into the abdominal cavity of a rodent?