SCYM Immunophenotyping 3

Question 1

Which immunophenotypic pattern on flow cytometry is most consistent with follicular lymphoma?

Accepted Answer

CD10+/BCL2+/CD19+/surface Ig+

Answer

Follicular lymphoma cells characteristically express CD10, BCL2, CD19, and surface immunoglobulin, reflecting their germinal center B-cell origin.

Question 2

In acute myeloid leukemia (AML), which marker combination suggests monocytic differentiation (AML-M5)?

Accepted Answer

CD13+/CD33+/CD64+/CD14+

Answer

Monocytic AML (FAB M5) is characterized by expression of myeloid markers plus monocyte-associated antigens CD14 and CD64.

Question 3

Which surface marker helps distinguish mantle cell lymphoma from CLL when both diseases co-express CD5 and CD19?

Accepted Answer

CD23 negativity in MCL

Answer

Mantle cell lymphoma is typically CD23-negative, while CLL is CD23-positive, making this a key discriminating marker between these two CD5+ B-cell neoplasms.

Question 4

In immunophenotyping of T-cell acute lymphoblastic leukemia (T-ALL), which marker indicates early T-cell precursor (ETP-ALL) subtype?

Accepted Answer

CD34 and CD117 co-expression with absent CD5

Answer

ETP-ALL is defined by expression of stem cell/myeloid markers (CD34, CD117) with absent or dim CD5 and no CD1a, reflecting arrested early T-cell development.

Question 5

Which antigen is characteristically expressed on Reed-Sternberg cells of classical Hodgkin lymphoma as detected by flow cytometry or immunohistochemistry?

Accepted Answer

CD30 and CD15

Answer

Reed-Sternberg cells in classical Hodgkin lymphoma characteristically express CD30 (Ki-1 antigen) and CD15, while lacking most B-cell and T-cell markers.

(SCYM) Specialist in Cytometry Practice Test

(SCYM) Specialist in Cytometry Practice Test

SCYM Immunophenotyping 3