SCYM Principles of Flow Cytometry & Instrumentation

Question 1

What is the main principle behind flow cytometry?

Accepted Answer

Light scattering and fluorescence

Answer

Sound resonance

Answer

Color matching

Answer

Heat conductivity

Question 2

What role does the laser play in flow cytometry?

Accepted Answer

Excites fluorophores for detection

Answer

Freezes cells

Answer

Sorts colors

Answer

Captures sound

Question 3

Which component directs cells into single file?

Accepted Answer

Hydrodynamic focusing

Answer

Rotary blade

Answer

Vacuum line

Answer

Electric coil

Question 4

What is forward scatter (FSC) an indicator of?

Accepted Answer

Cell size

Answer

Cell granularity

Answer

DNA content

Answer

Voltage level

Question 5

What does side scatter (SSC) measure?

Accepted Answer

Cell complexity/granularity

Answer

Voltage fluctuations

Answer

Cell rotation

Answer

Sound frequency

Question 6

Which detectors are used to measure fluorescence?

Accepted Answer

Photomultiplier tubes

Answer

Laser diodes

Answer

Thermometers

Answer

Spectrum analyzers

Question 7

What is the function of optical filters?

Accepted Answer

Isolate light wavelengths

Answer

Control humidity

Answer

Neutralize particles

Answer

Diffuse air

Question 8

Why is fluidics important in flow cytometry?

Accepted Answer

Aligns cells in flow

Answer

Regulates air

Answer

Applies pressure

Answer

Stabilizes temperature

Question 9

Which type of light is typically used in cytometry lasers?

Accepted Answer

Visible spectrum lasers (e.g., blue, red)

Answer

Infrared only

Answer

UV only

Answer

Gamma rays