SCYM Data Acquisition & Analysis

Question 1

What is gating in flow cytometry analysis?

Accepted Answer

Isolating data subsets

Answer

Amplifying data

Answer

Fixing light scatter

Answer

Suppressing graphs

Question 2

Which type of plot is most commonly used for fluorescence data?

Accepted Answer

Dot plot

Answer

Pie chart

Answer

Histogram

Answer

Line graph

Question 3

What does mean fluorescence intensity (MFI) indicate?

Accepted Answer

Expression level

Answer

Voltage strength

Answer

Cell size

Answer

Laser temperature

Question 4

Which software is commonly used for cytometry analysis?

Accepted Answer

FlowJo

Answer

Photoshop

Answer

Excel

Answer

Illustrator

Question 5

Why is compensation necessary in flow cytometry?

Accepted Answer

Remove spectral overlap

Answer

Brighten colors

Answer

Add contrast

Answer

Fix background

Question 6

What is a fluorescence minus one (FMO) control?

Accepted Answer

Sample missing one dye

Answer

All-inclusive dye set

Answer

Antibody marker

Answer

Calibration solution

Question 7

What type of scale is often used for displaying flow data?

Accepted Answer

Logarithmic

Answer

Linear

Answer

Percentage

Answer

Ordinal

Question 8

Which parameter represents signal intensity?

Accepted Answer

Fluorescence area

Answer

Time

Answer

Color hue

Answer

Scatter width

Question 9

Why are doublet discrimination tools used?

Accepted Answer

Eliminate cell clumps

Answer

Highlight nuclei

Answer

Increase noise

Answer

Change marker labels