In cytometry, what is the definition of 'median fluorescence intensity' (MFI), and why is it preferred over mean in skewed distributions?
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A
MFI is the most frequent value; it is preferred because it is computationally simple
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B
MFI is the middle value of ranked events; it is resistant to outliers and skewed tails
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C
MFI is the average intensity across all events; it captures total fluorescence accurately
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D
MFI is the geometric mean; it normalizes log-scale data automatically