When performing RT-PCR, why must random hexamer primers be used in the reverse transcription step instead of oligo-dT primers for fragmented or degraded RNA samples?
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A
Random hexamers prime throughout the RNA, capturing sequences even when poly-A tails are degraded
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B
Oligo-dT primers are more expensive and less available
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C
Random hexamers have higher Tm values than oligo-dT primers
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D
Oligo-dT primers inhibit reverse transcriptase activity