Free PCR Sample Preparation & Nucleic Acid Extraction Questions and Answers

Question 1

What is the goal of nucleic acid extraction?

Accepted Answer

To isolate DNA or RNA from samples

Answer

To stain the DNA

Answer

To dry the sample

Answer

To denature proteins

Question 2

Why must samples be free of contaminants before PCR?

Accepted Answer

They can inhibit enzyme activity

Answer

They improve binding

Answer

They help visualize DNA

Answer

They increase cycle number

Question 3

Which chemical helps lyse cells during DNA extraction?

Accepted Answer

Detergent (SDS)

Answer

Ethanol

Answer

Buffer only

Answer

RNAse

Question 4

What is the purpose of using proteinase K in DNA extraction?

Accepted Answer

Degrades proteins in the sample

Answer

Amplifies DNA

Answer

Stains DNA

Answer

Cuts RNA

Question 5

What role does ethanol play in DNA extraction?

Accepted Answer

Precipitates and isolates DNA

Answer

Dissolves DNA

Answer

Breaks RNA

Answer

Extracts proteins

Question 6

Why is centrifugation used in DNA extraction?

Accepted Answer

To pellet and separate DNA

Answer

To cool the sample

Answer

To activate enzymes

Answer

To measure pH

Question 7

Which sample type is commonly used for PCR?

Accepted Answer

Blood or tissue

Answer

Wood

Answer

Glass

Answer

Plastic

Question 8

What is the role of a lysis buffer?

Accepted Answer

Disrupts membranes and protects nucleic acids

Answer

Color the sample

Answer

Neutralize acids

Answer

Cuts proteins

Question 9

How is RNA contamination prevented in DNA extraction?

Accepted Answer

Add RNAse enzyme

Answer

Use ethanol

Answer

Heat at 90°C

Answer

Use pH strips