PCR PCR Applications & Clinical Diagnostics

Question 1

Which PCR application is most commonly used in US clinical laboratories to detect SARS-CoV-2?

Accepted Answer

RT-PCR (reverse transcription PCR)

Answer

Standard endpoint PCR

Answer

Allele-specific PCR

Answer

Inverse PCR

Question 2

In oncology diagnostics, which PCR-based method is used to detect minimal residual disease (MRD) in leukemia patients?

Accepted Answer

Quantitative real-time PCR (qPCR)

Answer

Nested PCR

Answer

Colony PCR

Answer

Asymmetric PCR

Question 3

Which PCR technique is used to identify single nucleotide polymorphisms (SNPs) associated with drug metabolism in pharmacogenomics?

Accepted Answer

Allele-specific PCR

Answer

Long-range PCR

Answer

Inverse PCR

Answer

Ligation-mediated PCR

Question 4

In forensic DNA analysis in the US, which PCR-based technique is used to generate a DNA profile for identity comparison?

Accepted Answer

STR (Short Tandem Repeat) PCR analysis

Answer

Digital PCR

Answer

Multiplex ligation-dependent probe amplification

Answer

Restriction fragment length polymorphism

Question 5

Which PCR application is used to diagnose genetic disorders prenatally by analyzing cell-free fetal DNA in maternal blood?

Accepted Answer

Non-invasive prenatal testing (NIPT) using qPCR

Answer

In situ PCR

Answer

Hot-start PCR

Answer

Reverse complement PCR

Question 6

For food safety testing in the US, which PCR method allows detection of multiple pathogens such as Salmonella and E. coli simultaneously in a single reaction?

Accepted Answer

Multiplex PCR

Answer

Touch-down PCR

Answer

Booster PCR

Answer

Isothermal PCR