ALAT Facility Operations and Sanitation 5

Question 1

An animal room has a persistent ammonia odor despite regular cage changes. What is the most likely corrective action?

Accepted Answer

Increase cage change frequency or improve ventilation

Answer

Persistent ammonia buildup indicates that cage change frequency or room ventilation is insufficient to remove waste-derived gases.

Question 2

What is the function of a biological safety cabinet (BSC) Class II Type A2 in an animal facility?

Accepted Answer

To protect personnel, the environment, and the product from contamination

Answer

A Class II Type A2 BSC provides personnel, environmental, and product protection by recirculating HEPA-filtered air within the cabinet.

Question 3

Which disinfectant class is considered most effective against non-enveloped viruses in animal facilities?

Accepted Answer

Aldehydes such as glutaraldehyde or accelerated hydrogen peroxide

Answer

Non-enveloped viruses are more resistant to disinfectants, and aldehydes or accelerated hydrogen peroxide products are among the most effective against them.

Question 4

What is the purpose of a sentinel animal program in a laboratory animal facility?

Accepted Answer

To monitor the health status of a colony by exposing specific animals to potential pathogens

Answer

Sentinel animals are exposed to dirty bedding or other colony materials so that their health status reflects the overall disease status of the room or colony.

Question 5

In a negative-pressure isolation room, air flows in which direction relative to the corridor?

Accepted Answer

From the corridor into the room

Answer

Negative-pressure rooms draw air inward from the corridor, preventing potentially contaminated room air from escaping and protecting the surrounding environment.

ALAT - Assistant Laboratory Animal Technician Practice Test

ALAT - Assistant Laboratory Animal Technician Practice Test

ALAT Facility Operations and Sanitation 5