ALAT Facility Environment and Management 5

Question 1

Which of the following is an example of environmental enrichment for laboratory mice that addresses their natural burrowing behavior?

Accepted Answer

Adding nesting material such as cotton nestlets

Answer

Nesting material satisfies the innate burrowing and nesting instincts of mice, reducing stress and improving welfare.

Question 2

What is the primary reason laboratory animal facilities use HEPA filters in their ventilation systems?

Accepted Answer

To remove particulates and pathogens from the air supply

Answer

HEPA filters capture ≥99.97% of particles ≥0.3 µm, including bacteria and spores, protecting animals from airborne pathogens.

Question 3

A rodent facility experiences an unexplained increase in pup mortality. Which environmental factor should be investigated FIRST?

Accepted Answer

Temperature fluctuations in the breeding room

Answer

Temperature extremes, especially cold drafts or heat spikes, are a leading environmental cause of neonatal mortality in rodents.

Question 4

Under current Guide recommendations, which of the following practices is acceptable for social species such as rabbits when housing is concerned?

Accepted Answer

Pair or group housing whenever scientifically and socially appropriate

Answer

The Guide emphasizes that social species should be housed in pairs or groups unless there is scientific or veterinary justification for single housing.

Question 5

When performing a room changeover after removing one mouse strain and before introducing another, what step is ESSENTIAL to prevent cross-contamination?

Accepted Answer

Thorough cleaning and disinfection of all surfaces and equipment

Answer

Complete cleaning and disinfection eliminates residual pathogens, allergens, and biological material before a new animal group is introduced.

ALAT - Assistant Laboratory Animal Technician Practice Test

ALAT - Assistant Laboratory Animal Technician Practice Test

ALAT Facility Environment and Management 5