ALAT Facility Environment and Management 3

Question 1

Which of the following is a SECONDARY containment measure for infectious agents in an animal biosafety level 2 (ABSL-2) facility?

Accepted Answer

The animal room itself (physical structure)

Answer

The animal room is considered secondary containment; primary containment includes the cage and BSC used during procedures.

Question 2

When decontaminating an animal room after an outbreak, which agent is considered effective against most bacterial, viral, and fungal pathogens on hard non-porous surfaces?

Accepted Answer

Diluted bleach (sodium hypochlorite)

Answer

Sodium hypochlorite (bleach) at appropriate dilutions is a broad-spectrum disinfectant effective against bacteria, viruses, and fungi on hard surfaces.

Question 3

What is the recommended maximum noise level in animal holding rooms according to the Guide?

Accepted Answer

85 dB

Answer

The Guide recommends keeping noise levels below 85 dB to prevent auditory and physiological stress in laboratory animals.

Question 4

A vivarium receives a shipment of new animals. What is the standard practice before introducing them to existing colonies?

Accepted Answer

Quarantine and health-monitor them in a separate area

Answer

Quarantine allows health monitoring and pathogen screening to prevent introduction of disease into established colonies.

Question 5

Which of the following environmental factors has the GREATEST direct influence on reproductive performance in laboratory rodents?

Accepted Answer

Photoperiod (light cycle)

Answer

Photoperiod strongly regulates reproductive hormones and cycles in rodents; irregular lighting disrupts breeding performance.

ALAT - Assistant Laboratory Animal Technician Practice Test

ALAT - Assistant Laboratory Animal Technician Practice Test

ALAT Facility Environment and Management 3