ALAT Breeding and Reproduction 2

Question 1

What is the typical estrous cycle length for a mouse?

Accepted Answer

4-5 days

Answer

The mouse estrous cycle is approximately 4-5 days with four stages: proestrus, estrus, metestrus, and diestrus.

Question 2

What is a vaginal plug and what does it indicate in mice?

Accepted Answer

A coagulated mass of seminal fluid indicating mating has occurred

Answer

A vaginal plug is coagulated seminal fluid that forms after mating. Checking for plugs each morning is standard for detecting mating in timed breeding.

Question 3

What is the typical litter size for laboratory mice?

Accepted Answer

6-12 pups

Answer

Laboratory mice typically produce 6-12 pups, varying by strain. C57BL/6 average 6-8 pups; CD-1 outbred may produce 10-14.

Question 4

What is the Whitten effect in mouse breeding?

Accepted Answer

Male pheromones synchronize estrous cycles in group-housed females

Answer

The Whitten effect occurs when male pheromones synchronize and stimulate estrous cycling in group-housed females within 3 days.

Question 5

At what age are mouse pups typically weaned?

Accepted Answer

21 days

Answer

Mouse pups are typically weaned at 21 days when they can eat solid food and drink independently. They should be separated by sex.

ALAT - Assistant Laboratory Animal Technician Practice Test

ALAT - Assistant Laboratory Animal Technician Practice Test

ALAT Breeding and Reproduction 2