Microbial Identification & Classification Flashcards

Which staining technique is commonly used to differentiate bacterial species?

Answer: Gram stain

The Gram stain differentiates bacteria based on the structure of their cell walls, classifying them as Gram-positive or Gram-negative.

What is the primary difference between prokaryotic and eukaryotic microorganisms?

Answer: Absence of a nucleus in prokaryotes

Prokaryotic cells, such as bacteria, lack a nucleus, whereas eukaryotic cells, such as fungi and protozoa, have a defined nucleus.

Which molecular technique is commonly used for bacterial identification?

Answer: PCR

Polymerase Chain Reaction (PCR) amplifies specific DNA sequences, allowing for rapid and accurate bacterial identification.

Which type of bacteria lacks a cell wall?

Answer: Mycoplasma

Mycoplasma species lack a cell wall, making them resistant to antibiotics that target cell wall synthesis, such as beta-lactams.

Which classification system is commonly used to identify microorganisms?

Answer: Bergey’s Manual

The Bergey’s Manual of Systematic Bacteriology is widely used for classifying and identifying bacterial species based on morphological, biochemical, and genetic characteristics.

What is the main characteristic of facultative anaerobes?

Answer: Can survive with or without oxygen

Facultative anaerobes can survive in both aerobic and anaerobic environments, switching between aerobic respiration and fermentation as needed.