Laboratory Science Lab Safety and Equipment 2

Question 1

What is the correct technique for pipetting a caustic chemical to prevent accidental ingestion?

Accepted Answer

Mechanical pipette aid or bulb

Answer

Mechanical pipette aids or bulbs must always be used; mouth pipetting is strictly prohibited in any laboratory setting.

Question 2

Which autoclave cycle parameter is standard for sterilizing aqueous solutions?

Accepted Answer

121°C for 15 minutes at 15 psi

Answer

Standard autoclave sterilization uses 121°C (250°F) at 15 psi for 15 minutes to ensure destruction of all microbial life including spores.

Question 3

What does SDS stand for in laboratory chemical safety?

Accepted Answer

Safety Data Sheet

Answer

A Safety Data Sheet (SDS) provides detailed information about a chemical's properties, hazards, handling, storage, and emergency procedures.

Question 4

When using a centrifuge, what is the most important safety precaution?

Accepted Answer

Balance opposing tubes with equal mass

Answer

Balancing opposing tubes with equal mass prevents rotor imbalance, which can cause vibration, mechanical failure, or catastrophic rotor destruction.

Question 5

Which waste container is appropriate for disposing of sharps in a clinical laboratory?

Accepted Answer

Rigid puncture-resistant sharps container

Answer

Sharps must be disposed of in rigid, puncture-resistant sharps containers to prevent needlestick injuries during handling and transport.

Laboratory Science Practice Test