KAPS Pharmaceutical Chemistry and Analysis 3

Question 1

Which metabolic reaction is considered a Phase I biotransformation reaction?

Accepted Answer

Oxidation via CYP450

Answer

CYP450-mediated oxidation is a Phase I reaction that introduces or unmasks a functional group on the drug molecule.

Question 2

In UV-Vis spectroscopy, Beer-Lambert's law states that absorbance is directly proportional to:

Accepted Answer

Concentration and path length

Answer

Beer-Lambert's law: A = εcl, where absorbance is proportional to molar absorptivity, concentration, and path length.

Question 3

Which functional group undergoes hydrolysis most readily under aqueous conditions, making it a common instability site in drugs?

Accepted Answer

Ester

Answer

Ester bonds are susceptible to hydrolysis by water (acid- or base-catalyzed), making esters a frequent degradation site in pharmaceuticals.

Question 4

What does a positive ninhydrin test indicate in the analysis of pharmaceutical compounds?

Accepted Answer

Presence of primary or secondary amines (amino acids)

Answer

Ninhydrin reacts with primary and secondary amines to produce a purple color (Ruhemann's purple), used to detect amino acids.

Question 5

Which parameter in chromatography is defined as the ratio of the time a solute spends in the stationary phase to the time it spends in the mobile phase?

Accepted Answer

Retention factor (k)

Answer

The retention factor k = (tR - tM) / tM, representing the ratio of time spent in stationary versus mobile phase.

(KAPS) Knowledge Assessment of Pharmaceutical Sciences Practice Test

(KAPS) Knowledge Assessment of Pharmaceutical Sciences Practice Test

KAPS Pharmaceutical Chemistry and Analysis 3