HT Microtomy & Staining 2

Question 1

Which microtome component is adjusted to control section thickness?

Accepted Answer

Micrometer handwheel

Answer

The micrometer handwheel on a rotary microtome controls the precise thickness advance per cutting stroke.

Question 2

A section shows a 'venetian blind' artifact with alternating thick and thin bands. What is the most likely cause?

Accepted Answer

Vibrating knife or block

Answer

Venetian blind artifact (chatter) is caused by vibration of the knife or block holder during cutting.

Question 3

What is the purpose of the anti-roll plate on a cryostat microtome?

Accepted Answer

To keep the section flat as it is cut

Answer

The anti-roll plate holds the section flat as it exits the knife edge, preventing it from curling.

Question 4

Masson's trichrome stain differentiates collagen from muscle using which color combination?

Accepted Answer

Collagen blue/green, muscle red

Answer

In Masson's trichrome, collagen stains blue or green while muscle fibers stain red.

Question 5

Which stain is used specifically to demonstrate amyloid deposits in tissue sections?

Accepted Answer

Congo red

Answer

Congo red stains amyloid deposits orange-red and shows apple-green birefringence under polarized light.

(HT) Histotechnician Certification Practice Test