HT Microtomy & Staining

Question 1

What is the purpose of microtomy in histology?

Accepted Answer

To cut tissue into thin slices for viewing under a microscope.

Answer

To remove excess moisture from tissue.

Answer

To stain tissue samples.

Answer

To prepare tissues for storage.

Question 2

Which of the following factors affects the quality of tissue sections during microtomy?

Accepted Answer

The cutting speed and knife quality.

Answer

Only the tissue type.

Answer

Room temperature.

Answer

Tissue age.

Question 3

What is the most common method of tissue staining in histology?

Accepted Answer

Hematoxylin and eosin (H&E) stain

Answer

Giemsa stain

Answer

Silver stain

Answer

Gram stain

Question 4

What does hematoxylin stain in tissue samples?

Accepted Answer

Nuclei

Answer

Cytoplasm

Answer

Extracellular matrix

Answer

Lipids

Question 5

Which of the following stains is commonly used for demonstrating collagen fibers?

Accepted Answer

Masson's trichrome

Answer

Hematoxylin

Answer

Silver nitrate

Answer

Carbolfuchsin

Question 6

What is the role of eosin in tissue staining?

Accepted Answer

It stains the cytoplasm pink to red.

Answer

It stains nuclei blue.

Answer

It stains connective tissue fibers.

Answer

It stains muscle fibers.

Question 7

What is the significance of using a cryostat in histology?

Accepted Answer

It freezes tissues for sectioning.

Answer

It stains tissues.

Answer

It fixes tissues.

Answer

It processes tissues in paraffin.

Question 8

What is the purpose of special staining techniques in histology?

Accepted Answer

To visualize specific tissue components or microorganisms.

Answer

To stain all cell types equally.

Answer

To enhance the contrast between tissues.

Answer

To preserve tissue architecture.

Question 9

What is the primary purpose of immunohistochemistry (IHC) in histology?

Accepted Answer

To detect specific proteins using antibodies.

Answer

To stain for cytoplasm.

Answer

To visualize the tissue structure.

Answer

To stain for lipids.