HT Tissue Processing

Question 1

What is the first step in tissue processing?

Accepted Answer

Fixation

Answer

Embedding

Answer

Dehydration

Answer

Clearing

Question 2

Why is tissue dehydration important in processing?

Accepted Answer

It allows tissue to be infiltrated with embedding media.

Answer

It prevents tissue hardening.

Answer

It removes lipids.

Answer

It facilitates staining.

Question 3

What is the purpose of clearing in tissue processing?

Accepted Answer

To make tissue transparent.

Answer

To remove the fixative.

Answer

To prepare tissues for embedding.

Answer

To harden tissue for sectioning.

Question 4

Which embedding media is commonly used in tissue processing?

Accepted Answer

Paraffin wax

Answer

Celloidin

Answer

Resin

Answer

Glycol

Question 5

How does tissue fixation help preserve the sample?

Accepted Answer

By preventing cellular breakdown.

Answer

By removing water.

Answer

By maintaining cellular integrity.

Answer

By preventing bacterial growth.

Question 6

What is the role of paraffin wax in tissue embedding?

Accepted Answer

It preserves the cellular structure.

Answer

It prevents the tissue from shrinking.

Answer

It makes the tissue more elastic.

Answer

It increases tissue transparency.

Question 7

What does over-dehydration of tissue lead to?

Accepted Answer

Distortion of tissue structure.

Answer

Better tissue clarity.

Answer

Improved embedding.

Answer

No effect on tissue quality.

Question 8

Which of the following is true regarding tissue sectioning?

Accepted Answer

Sections must be cut very thin for microscopic examination.

Answer

Thicker sections provide better clarity.

Answer

The sectioning process does not affect the tissue's appearance.

Answer

Thinner sections can be too fragile to handle.

Question 9

What is the common temperature used for paraffin embedding?

Accepted Answer

60-65°C

Answer

35-40°C

Answer

70-75°C

Answer

50-55°C