HT Microtomy & Sectioning Techniques 2

Question 1

What is the most common cause of 'chatter' artifact in paraffin sections?

Accepted Answer

Dull microtome knife

Answer

A dull or nicked knife blade is the most common cause of chatter, producing alternating thick and thin bands across the section.

Question 2

Which microtome type is most appropriate for cutting large, undecalcified bone specimens embedded in plastic (methacrylate)?

Accepted Answer

Sliding microtome

Answer

The sliding microtome, with its heavy construction and long knife stroke, is best suited for hard specimens like undecalcified bone in plastic.

Question 3

When sectioning a ribbon of paraffin sections, the sections curl away from the knife. What adjustment should be made?

Accepted Answer

Increase the clearance angle

Answer

Increasing the clearance (back) angle of the knife reduces friction on the bevel side and helps the ribbon lie flat.

Question 4

At what temperature should a water bath typically be maintained for floating paraffin sections?

Accepted Answer

5–10°C below the paraffin melting point

Answer

The floatation water bath is kept 5–10°C below the paraffin's melting point to allow wrinkles to relax without melting the section.

Question 5

A histotechnician notices that sections consistently compress along the cutting axis. Which action best corrects this?

Accepted Answer

Hone and strop the knife

Answer

Compression is most often caused by a dull knife; honing and stropping restores the cutting edge and reduces tissue compression.

(HT) Histotechnician Certification Practice Test