HTL Immunohistochemistry & Special Stains

Question 1

What is the primary purpose of immunohistochemistry (IHC) in histotechnology?

Accepted Answer

To detect specific proteins and antigens in tissue

Answer

To stain the entire tissue section

Answer

To remove pathogens from tissue

Answer

To measure tissue thickness

Question 2

What is the primary advantage of using special stains in histology?

Accepted Answer

To selectively stain specific tissue components for better visualization

Answer

To stain all tissue components equally

Answer

To eliminate the need for tissue preservation

Answer

To increase tissue sample size

Question 3

What is the most commonly used marker in immunohistochemistry?

Accepted Answer

Antibodies that bind to specific antigens

Answer

Dye-based stains

Answer

Fluorescent dyes

Answer

Enzyme-based markers

Question 4

How does the biotin-avidin system function in immunohistochemistry?

Accepted Answer

It enhances signal detection by binding biotin-labeled antibodies

Answer

It reduces antibody binding

Answer

It stains all cell components

Answer

It is used to fix tissue samples

Question 5

What type of tissue components are best highlighted using periodic acid-Schiff (PAS) stain?

Accepted Answer

Carbohydrates and glycoproteins

Answer

Proteins

Answer

Nucleic acids

Answer

Lipids

Question 6

How does the use of DAB (diaminobenzidine) contribute to immunohistochemistry?

Accepted Answer

It produces a brown color at the site of antibody binding

Answer

It enhances the color of the tissue

Answer

It stains the nucleus blue

Answer

It highlights lipids

Question 7

Why is it important to use a positive control in immunohistochemistry?

Accepted Answer

To confirm the antibody binding is functional and reliable

Answer

To increase the sensitivity of the assay

Answer

To reduce tissue sample preparation time

Answer

To enhance the color intensity of the tissue

Question 8

What is the purpose of using immunofluorescence in histology?

Accepted Answer

To identify specific proteins or antigens in tissue

Answer

To use antibodies as fluorescent markers

Answer

To enhance the resolution of tissue samples

Answer

To stain the entire tissue sample

Question 9

How does immunohistochemistry contribute to cancer diagnosis?

Accepted Answer

By detecting cancerous markers and identifying cancer types

Answer

By providing information about tissue structure

Answer

By monitoring tumor size

Answer

By increasing the accuracy of imaging techniques