SCYM Cell Cycle & DNA Content Analysis 3

Question 1

Which dedicated software tool uses polynomial and Gaussian modeling algorithms specifically for automated cell cycle and DNA ploidy analysis?

Accepted Answer

ModFit LT

Answer

FCS Express scatter module

Answer

FlowJo proliferation module

Answer

Kaluza statistics editor

Question 2

What maximum CV for the G0/G1 peak is generally considered acceptable for clinical DNA ploidy analysis by flow cytometry?

Accepted Answer

Less than 5%

Answer

Less than 3%

Answer

Less than 8%

Answer

Less than 10%

Question 3

Which antibody marker best helps distinguish tetraploid G1 cells (4N) from true G2/M cells (also 4N) in DNA content analysis?

Accepted Answer

MPM-2 anti-phosphoprotein antibody

Answer

Anti-CD45 antibody

Answer

Annexin V

Answer

Anti-Ki-67 antibody

Question 4

In clinical oncology, a high S-phase fraction (SPF) detected by DNA content analysis in breast cancer is associated with:

Accepted Answer

More aggressive tumor behavior and worse prognosis

Answer

Improved disease-free survival

Answer

Hormone receptor positivity

Answer

Lower nuclear grade

Question 5

Which chemical agent is used to synchronize cells at the G1/S boundary by inhibiting ribonucleotide reductase?

Accepted Answer

Hydroxyurea

Answer

Nocodazole

Answer

Colchicine

Answer

Aphidicolin

Question 6

When performing DNA ploidy analysis on a fresh tumor biopsy, what is the purpose of including normal stromal cells within the same specimen as an internal reference?

Accepted Answer

To establish the diploid G1 peak position for accurate DNA index calculation

Answer

To improve the CV of the G1 DNA peak

Answer

To increase the total analyzable cell count

Answer

To identify and gate out debris particles

Question 7

A tumor sample with a measured DNA index (DI) of 1.5 is best classified as:

Accepted Answer

Hyperdiploid aneuploid tumor cells with increased DNA content

Answer

Normal diploid with standard DNA content

Answer

Hypodiploid cells undergoing apoptotic DNA loss

Answer

Tetraploid cells arrested in G2/M