PCAT Study Guide 2026

Everything you need to pass the PCAT exam in one place: the exam format, every topic to study, real practice questions with explanations, flashcards, and full-length practice tests. Free, no sign-up needed.

📋 PCAT Exam Format at a Glance

192
Questions
165 min
Time Limit
70.00%
Passing Score

📚 PCAT Topics to Study (69)

✍️ Sample PCAT Questions & Answers

1. Which of the following best describes the colligative property of boiling point elevation?
It depends only on the number of solute particles in solution

Boiling point elevation is a colligative property, meaning it depends on the number (not the identity) of dissolved solute particles per kilogram of solvent.

2. Resistance to beta-lactam antibiotics via production of beta-lactamase enzymes can be overcome by administering:
Clavulanic acid

Clavulanic acid is a beta-lactamase inhibitor that irreversibly binds and inactivates beta-lactamase, protecting co-administered beta-lactam antibiotics.

3. Which of the following organisms does not have mitochondria?
bacteria

Bacteria are prokaryotic organisms, meaning they lack membrane-bound organelles, including mitochondria. Instead of mitochondria, bacteria perform cellular respiration using enzymes embedded in their cell membrane. Amoeba, fungi, and protozoans are all eukaryotic organisms and therefore possess mitochondria for energy production.

4. Which of the following is equivalent to (x²y³)⁴ / (x³y²)?
x⁵y¹⁰

(x²y³)⁴ = x⁸y¹²; dividing by x³y² gives x⁵y¹⁰.

5. What is the net ATP yield from complete aerobic oxidation of one glucose molecule (using the malate-aspartate shuttle)?
30-32 ATP

Modern estimates calculate approximately 30-32 ATP net from aerobic glucose oxidation, accounting for the actual P/O ratios of NADH (~2.5 ATP) and FADH2 (~1.5 ATP) rather than the older theoretical values.

6. During meiosis II, which event separates sister chromatids?
Anaphase II

During Anaphase II, cohesins break down and sister chromatids are pulled to opposite poles of the cell.

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1. Learn with Flashcards → 2. Drill Practice Tests → 3. Take the Full Exam Simulation
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PCAT Study Guide 2026 — Exam Format, Topics & Practice Questions