PAP PAP Specimen Collection and Preparation

Question 1

What is the recommended instrument combination for collecting cells from the cervical transformation zone during a Pap test?

Accepted Answer

Endocervical brush combined with a spatula

Answer

The combination of an endocervical brush and spatula is recommended to sample both the ectocervix and the endocervical canal.

Question 2

How should a liquid-based cytology (LBC) specimen be processed immediately after collection?

Accepted Answer

Rinsed into a vial of preservative solution

Answer

In liquid-based cytology, the collection device is rinsed into a vial of preservative solution to create a uniform cell suspension.

Question 3

For how long before a Pap test should a patient avoid sexual intercourse to prevent specimen contamination?

Accepted Answer

48 hours

Answer

Patients are advised to avoid sexual intercourse for at least 48 hours before a Pap test to prevent contamination of the cervical specimen.

Question 4

Which fixative is used for conventional Pap smears to prevent air-drying artifact?

Accepted Answer

95% ethanol

Answer

95% ethanol (or a spray fixative containing alcohol) must be applied immediately to the smear to prevent cellular distortion from air-drying.

Question 5

At what point in the menstrual cycle is it ideal to schedule a Pap specimen collection?

Accepted Answer

Days 10-20 of the cycle

Answer

Mid-cycle (days 10-20) is optimal because cervical mucus is thinnest and cellular shedding is most representative of the transformation zone.

PAP - Papanicolaou Test Practice Test