CER - Certified Endoscope Reprocessor Endoscope Sterilization Methods Questions and Answers

Question 1

A reprocessing facility is validating its Ethylene Oxide (EtO) sterilization cycle for flexible endoscopes. Which of the following sets of parameters are all essential for achieving effective EtO sterilization?

Accepted Answer

Gas concentration, temperature, relative humidity, and exposure time

Answer

Effective Ethylene Oxide (EtO) sterilization depends on four critical parameters: gas concentration (typically 450 to 1200 mg/l), temperature (37 to 63°C), relative humidity (40 to 80%), and exposure time (1 to 6 hours). All four must be within the specified ranges for the cycle to be effective. While pressure and aeration time are parts of the overall process, they are not considered the four essential parameters for achieving sterility.

Question 2

A facility is considering a low-temperature sterilization method for its new heat-sensitive flexible duodenoscopes. They need a method that uses a vapor, is converted to a plasma state to enhance microbial kill, and breaks down into non-toxic byproducts like oxygen and water. Which sterilization method fits this description?

Accepted Answer

Vaporized Hydrogen Peroxide Gas Plasma

Answer

Vaporized Hydrogen Peroxide (VHP) Gas Plasma sterilization uses hydrogen peroxide vapor which is then excited by an electric field into a plasma state. This process generates free radicals that are highly effective at killing microorganisms. A key advantage is that at the end of the cycle, the byproducts are non-toxic water and oxygen, eliminating the need for lengthy aeration.

Question 3

According to ANSI/AAMI ST91, what is the recommended practice for endoscopes that have been processed using a liquid chemical sterilization (LCS) system but are not intended for immediate use?

Accepted Answer

They can be stored in the same manner as endoscopes that have undergone high-level disinfection.

Answer

ANSI/AAMI ST91 states that liquid chemically sterilized endoscopes intended for critical procedures should be used immediately. If not for immediate use, they can be stored in the same way as endoscopes that have undergone high-level disinfection (HLD), which involves thorough drying and storage in a clean, dedicated cabinet. They should not be stored with a claim of being patient-ready sterile.

Question 4

A reprocessing technician is using an Automated Endoscope Reprocessor (AER) that utilizes a peracetic acid-based system for liquid chemical sterilization. What is the primary mechanism by which peracetic acid inactivates microorganisms?

Accepted Answer

Oxidation of cell components

Answer

Peracetic acid is a highly biocidal oxidizer. Its primary mechanism of action is oxidation, where it denatures proteins, disrupts cell wall permeability, and oxidizes essential bonds in proteins and enzymes, leading to the shutdown of cellular function and death of the microorganism.

Question 5

Which of the following statements correctly distinguishes sterilization from high-level disinfection (HLD) in the context of endoscope reprocessing?

Accepted Answer

Sterilization provides a sterility assurance level (SAL) by eliminating all viable microorganisms, including large numbers of bacterial spores.

Answer

The key difference is the level of microbial kill. Sterilization is a validated process that renders a device free from all viable microorganisms, including high numbers of bacterial spores, achieving a specific sterility assurance level (SAL). High-level disinfection kills all vegetative microorganisms, mycobacteria, viruses, and fungi, but not necessarily large numbers of bacterial spores.

CER - Certified Endoscope Reprocessor Practice Test

CER - Certified Endoscope Reprocessor Practice Test

CER - Certified Endoscope Reprocessor Endoscope Sterilization Methods Questions and Answers