ASCP Tissue Processing

Question 1

What is the primary purpose of fixation in tissue processing?

Accepted Answer

To prevent autolysis and preserve tissue morphology

Answer

Fixation prevents autolysis and microbial decomposition by cross-linking proteins to preserve tissue architecture.

Question 2

Which fixative is considered the gold standard for routine histological processing?

Accepted Answer

10% neutral buffered formalin

Answer

10% neutral buffered formalin is the standard fixative because it preserves morphology well and is compatible with most downstream staining techniques.

Question 3

What is the purpose of the dehydration step in tissue processing?

Accepted Answer

To remove water before infiltration with paraffin

Answer

Dehydration removes water from tissue using ascending grades of alcohol so the tissue can be infiltrated with paraffin wax.

Question 4

Which reagent is commonly used as a clearing agent to remove alcohol before paraffin infiltration?

Accepted Answer

Xylene

Answer

Xylene is miscible with both alcohol and paraffin, making it the standard clearing agent in tissue processing.

Question 5

What processing artifact results from inadequate fixation before processing?

Accepted Answer

Autolysis

Answer

Inadequate fixation allows continued enzymatic activity, leading to autolysis and degraded tissue morphology.

ASCP - Histotechnician Test Practice Test

ASCP - Histotechnician Test Practice Test

ASCP Tissue Processing