ASCP Microtomy and Cryostat Techniques

Question 1

What is the standard section thickness used for routine paraffin H&E sections?

Accepted Answer

4–5 µm

Answer

1–2 µm

Answer

10–12 µm

Answer

20–25 µm

Question 2

What is the purpose of the water bath used during paraffin sectioning?

Accepted Answer

To float and flatten sections by removing compression wrinkles

Answer

To fix sections before mounting

Answer

To stain sections

Answer

To remove paraffin from sections

Question 3

What type of blade is used for cutting frozen sections on a cryostat?

Accepted Answer

Low-profile disposable blade

Answer

High-profile disposable blade

Answer

Carbide-tipped blade

Answer

Diamond blade

Question 4

What is the primary advantage of frozen sections over paraffin sections during intraoperative consultation?

Accepted Answer

Rapid turnaround time for immediate surgical decisions

Answer

Superior morphological detail

Answer

Better antigen preservation for IHC

Answer

Easier to cut thin sections

Question 5

What is 'chatter' in microtomy?

Accepted Answer

Parallel lines or waves across a section due to vibration

Answer

Tissue folding during cutting

Answer

Incomplete paraffin removal

Answer

Tissue tearing at edges

Question 6

What temperature range is a cryostat chamber maintained at for optimal sectioning of most tissues?

Accepted Answer

-15°C to -25°C

Answer

-5°C to -10°C

Answer

-30°C to -40°C

Answer

0°C to -5°C