ASCP Microtomy and Cryostat Techniques

Question 1

What is the standard section thickness used for routine paraffin H&E sections?

Accepted Answer

4–5 µm

Answer

Routine paraffin sections are cut at 4–5 µm to allow adequate light transmission and single-cell-layer evaluation.

Question 2

What is the purpose of the water bath used during paraffin sectioning?

Accepted Answer

To float and flatten sections by removing compression wrinkles

Answer

The water bath (typically 40–45°C) allows compressed sections to expand and flatten before being picked up on slides.

Question 3

What type of blade is used for cutting frozen sections on a cryostat?

Accepted Answer

Low-profile disposable blade

Answer

Low-profile disposable blades are used in cryostats because they fit the cryostat microtome head and are designed for frozen tissue.

Question 4

What is the primary advantage of frozen sections over paraffin sections during intraoperative consultation?

Accepted Answer

Rapid turnaround time for immediate surgical decisions

Answer

Frozen sections can be prepared in 10–15 minutes, allowing surgeons to receive immediate pathological guidance during an operation.

Question 5

What is 'chatter' in microtomy?

Accepted Answer

Parallel lines or waves across a section due to vibration

Answer

Chatter produces parallel lines across the section caused by vibration between the blade and the block during cutting.

ASCP - Histotechnician Test Practice Test