ASCP Laboratory Safety and Quality Control 2

Question 1

What is the permissible exposure limit (PEL) for formaldehyde established by OSHA?

Accepted Answer

0.75 ppm (8-hour TWA)

Answer

OSHA sets the PEL for formaldehyde at 0.75 ppm as an 8-hour time-weighted average, with a 2 ppm short-term exposure limit.

Question 2

When should an autoclave (steam sterilization) be used in the histology laboratory?

Accepted Answer

To decontaminate biohazardous waste and instruments

Answer

Autoclaving uses pressurized steam to sterilize biohazardous waste, contaminated instruments, and materials before disposal or reuse.

Question 3

What does a reagent QC failure on a negative control indicate?

Accepted Answer

Non-specific or background staining is present, suggesting a reagent or protocol problem

Answer

A positive signal on a negative control means non-specific staining is occurring, which could lead to false-positive results in patient samples.

Question 4

In the context of laboratory safety, what is the purpose of a Safety Data Sheet (SDS)?

Accepted Answer

To provide comprehensive hazard, handling, storage, and emergency information for chemical substances

Answer

SDS (formerly MSDS) documents provide detailed chemical hazard information including toxicity, flammability, PPE requirements, and spill/exposure procedures.

Question 5

What is the minimum required documentation for each H&E staining run in an accredited laboratory?

Accepted Answer

Reagent lot numbers, dates, technician ID, and control results

Answer

Accreditation standards require each staining run to be documented with reagent lot numbers, preparation/expiration dates, technician identification, and control staining results.

ASCP - Histotechnician Test Practice Test

ASCP - Histotechnician Test Practice Test

ASCP Laboratory Safety and Quality Control 2