ALAT ALAT Research Procedures and Techniques Questions and Answers 2

Question 1

Which anesthetic agent is commonly used as an injectable anesthetic combination in rodents for short procedures?

Accepted Answer

Ketamine and xylazine

Answer

Ketamine/xylazine is a widely used injectable anesthetic combination in rodents for short to medium-length procedures.

Question 2

What is the correct method to confirm a rodent is under adequate anesthesia before beginning a procedure?

Accepted Answer

Assess absence of response to a firm toe pinch

Answer

Absence of a withdrawal response to a firm toe (pedal) pinch is the standard test for adequate anesthetic depth.

Question 3

Which of the following best describes the purpose of using an analgesic in post-procedural animal care?

Accepted Answer

To relieve pain and improve animal welfare

Answer

Analgesics are administered post-procedurally to alleviate pain, supporting animal welfare and reducing stress-related confounds.

Question 4

What information should be recorded in a laboratory animal's procedure log?

Accepted Answer

Date, procedure, dose, route, volume, and technician performing the procedure

Answer

Complete procedure records must include the date, procedure type, substance administered (dose, route, volume), and who performed it.

Question 5

When collecting a blood sample via cardiac puncture in a mouse, this procedure is typically performed:

Accepted Answer

As a terminal procedure under anesthesia

Answer

Cardiac puncture is a terminal procedure performed under deep anesthesia because it is not survivable.

ALAT - Assistant Laboratory Animal Technician Practice Test