When using carbon dioxide (CO2) for euthanasia of a trapped small mammal, what fill rate is recommended by AVMA Guidelines to minimize distress?
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A
Fill the chamber to 100% CO2 concentration instantly before introducing the animal
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B
Displace 30–70% of the chamber volume per minute with CO2
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C
Introduce CO2 slowly at less than 10% chamber volume per minute to allow gradual sedation
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D
Pre-fill the chamber completely, then introduce the animal into the saturated environment