Specimen Collection & Handling Flashcards

Which analyte must be protected from light during handling?

Answer: Bilirubin

Bilirubin degrades with light exposure, so tubes are wrapped in foil or amber containers.

How long should an SST tube clot before centrifugation?

Answer: About 30 minutes

Allowing about 30 minutes ensures complete clotting before the gel barrier forms.

How should an ammonia specimen be transported?

Answer: On ice and delivered promptly

Ammonia rises quickly in vitro, so chilling and rapid delivery are required.

What is the correct skin prep step before drawing blood cultures?

Answer: Cleanse with antiseptic and allow it to dry completely

Full drying time is needed for antiseptic efficacy and to reduce contamination.

How should specimens be transported between facilities?

Answer: In sealed, leakproof secondary containers with a biohazard symbol

Biohazard rules require leakproof secondary containment and labeling.

Which specimen must be kept warm at about 37°C until separation?

Answer: Cold agglutinin testing

Chilling would cause cold agglutinins to bind red cells and give false results.

When performing a capillary fingerstick, what should be done with the first drop of blood?

Answer: Wipe it away

The first drop contains tissue fluid and alcohol that can contaminate the sample.