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MLPAO Microbiology and Parasitology Diagnostic Methods Flashcards

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Read the first 7 MLPAO Microbiology and Parasitology Diagnostic Methods flashcards as text
  1. Which colony appearance on Salmonella-Shigella (SS) agar suggests Salmonella?

    Answer: Black-centered colonies on SS agar

    Salmonella is a non-lactose fermenter that produces H2S, giving colorless colonies with black centers on SS agar.

  2. Which blood parasite is identified on thin blood film by banana-shaped (crescent) gametocytes?

    Answer: Plasmodium falciparum

    Crescent-shaped gametocytes are characteristic of Plasmodium falciparum.

  3. On Kirby-Bauer disk diffusion testing, which factor would most likely produce falsely small zones of inhibition?

    Answer: Inoculum heavier than a 0.5 McFarland standard

    An overly heavy inoculum overwhelms the antibiotic and reduces zone sizes.

  4. A CSF India ink preparation shows encapsulated budding yeast. Which organism is most likely?

    Answer: Cryptococcus neoformans

    Cryptococcus neoformans has a polysaccharide capsule that excludes India ink, creating a clear halo.

  5. Which test distinguishes Streptococcus pneumoniae from viridans streptococci?

    Answer: Optochin susceptibility and bile solubility

    S. pneumoniae is optochin susceptible and bile soluble, unlike viridans streptococci.

  6. Which is the best quality control practice when a new lot of culture media is received?

    Answer: Test with known positive and negative control organisms

    New media lots must be checked for sterility and for growth performance using known QC strains.

  7. Which protozoan trophozoite is characterized by a 'falling leaf' motility and a pear-shaped body with two nuclei in a stool wet mount?

    Answer: Giardia lamblia

    Giardia trophozoites are pear-shaped, binucleate with a sucking disc and show falling-leaf motility.

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MLPAO Microbiology and Parasitology Diagnostic Methods Flashcards — MLPAO Study Cards with Answers