ISAC Laboratory Safety and Procedures 4

Question 1

Which type of biological safety cabinet (BSC) is NOT appropriate for protecting the operator from aerosol exposure when handling infectious cytometry samples?

Accepted Answer

Horizontal laminar flow clean bench

Answer

Horizontal laminar flow clean benches blow air toward the operator and provide no personnel protection; they are for product protection only.

Question 2

What is the recommended contact time for a 10% bleach solution to effectively inactivate bloodborne pathogens on contaminated cytometer surfaces?

Accepted Answer

10 minutes

Answer

A 10% bleach solution requires a minimum 10-minute contact time to achieve effective inactivation of bloodborne pathogens on surfaces.

Question 3

A flow cytometry laboratory must keep Safety Data Sheets (SDS) readily accessible. Under GHS/HazCom 2012, how many sections does a compliant SDS contain?

Accepted Answer

16

Answer

GHS-aligned SDS documents contain exactly 16 standardized sections covering identification, hazards, composition, first aid, handling, and disposal.

Question 4

When performing propidium iodide (PI) staining for viability in flow cytometry, what specific hazard requires attention during disposal?

Accepted Answer

PI is a known mutagen requiring disposal as hazardous chemical waste

Answer

Propidium iodide is a DNA intercalator and suspected mutagen; waste containing PI must be disposed of as hazardous chemical waste, not poured down the drain.

Question 5

Which eye protection is adequate when working with Class 3B lasers in a flow cytometry facility?

Accepted Answer

Optical density (OD) 4+ laser safety eyewear matched to the laser wavelength

Answer

Class 3B laser work requires wavelength-specific laser safety eyewear with sufficient optical density (typically OD ≥4) to attenuate the beam to safe levels.

(ISAC) International Society for the Advancement of Cytometry Practice Test

(ISAC) International Society for the Advancement of Cytometry Practice Test

ISAC Laboratory Safety and Procedures 4