HTL Tissue Preparation & Processing

Question 1

What is the primary purpose of tissue fixation in histology?

Accepted Answer

To prevent the tissue from decaying and preserve its structure

Answer

To shrink the tissue for easier handling

Answer

To soften the tissue for better sectioning

Answer

To remove water from the tissue

Question 2

Which of the following is a common fixative used in tissue processing?

Accepted Answer

Formalin

Answer

Ethanol

Answer

Acetone

Answer

Xylene

Question 3

Why is tissue dehydration necessary in histology?

Accepted Answer

To prepare the tissue for embedding in paraffin

Answer

To preserve the tissue's natural color

Answer

To enhance the tissue's strength

Answer

To make the tissue more flexible

Question 4

What is the purpose of embedding tissue in paraffin?

Accepted Answer

To facilitate easier cutting and maintain tissue integrity

Answer

To prevent the tissue from becoming too hard

Answer

To allow the tissue to expand during sectioning

Answer

To keep the tissue at room temperature

Question 5

Which staining method is commonly used to differentiate between different tissue types?

Accepted Answer

Hematoxylin and eosin (H&E) stain

Answer

Silver stain

Answer

Periodic acid-Schiff (PAS) stain

Answer

Giemsa stain

Question 6

Why is sectioning important in histology?

Accepted Answer

To prepare the tissue for microscopic examination

Answer

To remove excess embedding material

Answer

To enhance the color of the tissue

Answer

To preserve the tissue for long-term storage