HTL Staining Techniques

Question 1

What is the main purpose of histological staining?

Accepted Answer

To provide contrast for detailed examination of tissue structures

Answer

To make tissue samples more rigid

Answer

To increase the size of tissue samples

Answer

To remove unnecessary tissue components

Question 2

Which of the following is a common use for hematoxylin in tissue staining?

Accepted Answer

To stain cell nuclei blue or purple

Answer

To stain the cytoplasm pink

Answer

To highlight collagen fibers

Answer

To stain the intercellular matrix yellow

Question 3

What is the purpose of eosin in the H&E staining technique?

Accepted Answer

To stain the cytoplasm and red blood cells pink or red

Answer

To stain cell nuclei blue

Answer

To stain collagen fibers brown

Answer

To stain the background tissue colorless

Question 4

Which staining method is used to highlight bacterial organisms in tissue samples?

Accepted Answer

Gram stain

Answer

Hematoxylin and eosin stain

Answer

Acid-fast stain

Answer

Silver stain

Question 5

What is the main purpose of the periodic acid-Schiff (PAS) stain?

Accepted Answer

To detect glycogen and mucins in tissues

Answer

To stain collagen fibers blue

Answer

To highlight cell nuclei green

Answer

To differentiate bacterial species

Question 6

What is the function of the Wright-Giemsa stain?

Accepted Answer

To identify blood cells and their morphology

Answer

To detect fungal infections in tissue samples

Answer

To detect collagen and elastic fibers

Answer

To stain the cell membrane only