Dental Instrument Sterilization Flashcards

How often does the CDC recommend performing biological monitoring (spore testing) of dental office sterilizers?

Answer: At least weekly

The CDC recommends biological monitoring at least weekly. A positive result means the sterilizer must be removed from service.

What is the primary purpose of using an enzyme-based cleaner during the pre-cleaning phase of instrument reprocessing?

Answer: To break down organic material such as blood and protein

Enzyme-based cleaners contain proteases and lipases that actively break down organic matter on instruments, facilitating thorough cleaning before sterilization.

What is the correct procedure when a biological indicator test comes back positive?

Answer: Remove the sterilizer from service and investigate the cause

A positive BI means sterilization failure. The unit must be removed from service, the cause investigated, and items processed since the last negative test recalled.

Which instrument packaging material is NOT acceptable for use in a steam autoclave?

Answer: Nylon/plastic pouches without paper backing

Closed nylon or plastic pouches without a paper or Tyvek backing do not allow steam penetration, which is essential for steam sterilization.

What is the maximum recommended storage time for sterilized packs stored under optimal conditions per event-related shelf life principles?

Answer: Indefinite if packaging integrity is maintained

Under event-related shelf life principles, sterilized packs remain sterile indefinitely as long as packaging remains intact, dry, and undamaged.

What temperature range is required for effective dry heat sterilization of dental instruments?

Answer: 320-340°F (160-171°C) for 60-120 minutes

Dry heat sterilization requires 320-340°F (160-171°C) for 60-120 minutes because dry heat transfers energy less efficiently than moist heat.