CTP Organ Preservation Techniques 3

Question 1

Which mechanism primarily drives reperfusion injury after cold ischemia?

Accepted Answer

Reactive oxygen species (ROS) burst from xanthine oxidase activation

Answer

Xanthine oxidase converts hypoxanthine to uric acid upon oxygen reintroduction, generating a burst of ROS that damages cell membranes.

Question 2

What is the main advantage of Custodiol HTK (histidine-tryptophan-ketoglutarate) solution over UW solution for cardiac preservation?

Accepted Answer

Lower viscosity allowing better coronary perfusion

Answer

HTK's low viscosity enables more uniform and complete coronary distribution compared to the more viscous UW solution.

Question 3

In the context of donation after circulatory death (DCD), what distinguishes 'warm ischemia time' from 'functional warm ischemia time'?

Accepted Answer

Functional warm ischemia begins when SBP <50 mmHg or SpO₂ <70%, not at circulatory arrest

Answer

Functional warm ischemia starts when perfusion becomes inadequate (SBP <50 or SpO₂ <70%), capturing the period of physiologically significant ischemia before arrest.

Question 4

Which kidney machine perfusion parameter most accurately predicts post-transplant function?

Accepted Answer

Resistive index (RI)

Answer

The resistive index reflects vascular resistance in the kidney and is the most validated perfusion parameter for predicting delayed graft function.

Question 5

Why is Celsior solution particularly favored for heart and lung preservation?

Accepted Answer

It contains high concentrations of histidine for buffering and mannitol as an osmotic agent

Answer

Celsior's histidine buffering capacity and mannitol-based osmolarity make it well-suited for thoracic organ preservation.

(CTP) Certified Transplant Preservationist Practice Test

(CTP) Certified Transplant Preservationist Practice Test

CTP Organ Preservation Techniques 3