CTP CTP Organ Viability Assessment & Machine Perfusion 2

Question 1

Which biomarker measured in perfusate during normothermic machine perfusion of a liver is used to assess biliary viability?

Accepted Answer

Bile production rate and composition

Answer

Bile production during normothermic perfusion indicates that cholangiocytes are viable and functioning, as biliary viability is critical for avoiding ischemic cholangiopathy post-transplant.

Question 2

In the context of DCD (donation after circulatory death) organ procurement, 'warm ischemia time' begins at:

Accepted Answer

Withdrawal of life-sustaining treatment and circulatory arrest until cold flush initiation

Answer

Warm ischemia time in DCD donation begins when perfusion becomes inadequate (typically at circulatory arrest after withdrawal of support) and ends when cold preservation flush starts.

Question 3

The LifePort Kidney Transporter uses which perfusion strategy during organ transport?

Accepted Answer

Continuous hypothermic pulsatile perfusion at 4°C

Answer

The LifePort uses continuous hypothermic pulsatile perfusion at approximately 4°C, allowing the kidney to be transported while simultaneously being machine perfused to minimize ischemic injury.

Question 4

Which AST/ALT level threshold in normothermic liver perfusate is commonly used as a viability cutoff before accepting the organ for transplantation?

Accepted Answer

AST <100 IU/L at steady state during perfusion

Answer

An AST below approximately 100 IU/L at steady state during normothermic perfusion suggests acceptable hepatocyte injury and is used as part of the viability criteria for liver acceptance.

Question 5

What does the term 'subnormothermic machine perfusion' (SNMP) refer to in organ preservation?

Accepted Answer

Perfusion at intermediate temperatures (20–25°C) to partially restore metabolism while limiting rewarming injury

Answer

Subnormothermic machine perfusion operates at 20–25°C, an intermediate temperature that partially restores aerobic metabolism and allows some cellular repair while still reducing the high metabolic demands of full normothermia.

(CTP) Certified Transplant Preservationist Practice Test

(CTP) Certified Transplant Preservationist Practice Test

CTP CTP Organ Viability Assessment & Machine Perfusion 2