CSC Drug & Alcohol Testing Protocols 2 — Questions and Answers
Question 1: Under DOT regulations, what is the minimum volume of urine required for a primary specimen in a federal workplace drug test?
- 30 mL (Correct answer)
- 45 mL
- 60 mL
- 15 mL
Correct answer: 30 mL
DOT requires a minimum of 30 mL of urine for the primary specimen in a split-specimen collection.
Question 2: A donor's urine temperature reads 92°F immediately after collection. What should the collector do?
- Report the specimen as out-of-range and follow shy bladder procedures
- Accept the specimen since it is within the acceptable range (Correct answer)
- Discard the specimen and restart the collection
- Add warm water to bring temperature into range
Correct answer: Accept the specimen since it is within the acceptable range
The acceptable temperature range is 90–100°F (32–38°C), so 92°F falls within the valid range and the specimen should be accepted.
Question 3: Which federal agency's 49 CFR Part 40 establishes the procedures for drug and alcohol testing in transportation industries?
- DEA
- DOT (Correct answer)
- SAMHSA
- OSHA
Correct answer: DOT
The Department of Transportation (DOT) issued 49 CFR Part 40, which governs workplace drug and alcohol testing procedures.
Question 4: During a direct observation collection, who is authorized to observe the donor providing a urine specimen?
- Any certified collector regardless of gender
- An observer of the same gender as the donor (Correct answer)
- A supervisor of the donor
- A medical review officer
Correct answer: An observer of the same gender as the donor
Direct observation must be conducted by a collector or observer of the same gender as the donor to preserve dignity and privacy.
Question 5: What action must a collector take if a donor refuses to sign the custody and control form (CCF)?
- Discard the specimen and end the collection
- Note the refusal on the CCF and treat it as a refusal to test (Correct answer)
- Sign on the donor's behalf and proceed
- Contact law enforcement immediately
Correct answer: Note the refusal on the CCF and treat it as a refusal to test
A donor's refusal to sign the CCF is documented by the collector and treated as a refusal to test, which has the same consequences as a positive result under DOT rules.
Question 6: What is the shelf-life concern that makes proper specimen storage temperature critical before shipment to the laboratory?
- High temperatures accelerate bacterial growth that can alter creatinine levels (Correct answer)
- Cold temperatures cause the specimen cup to crack
- Room temperature causes the colorimetric strip to fade
- Freezing destroys the specimen seal integrity
Correct answer: High temperatures accelerate bacterial growth that can alter creatinine levels
Bacterial growth in specimens stored at elevated temperatures can produce false creatinine and pH results, potentially masking adulteration or substitution.
Question 7: Under DOT rules, which of the following constitutes a 'shy bladder' situation?
- A donor produces exactly 30 mL of urine
- A donor is unable to provide a sufficient specimen within the required timeframe (Correct answer)
- A donor requests a same-gender collector
- A donor's specimen temperature is below 90°F
Correct answer: A donor is unable to provide a sufficient specimen within the required timeframe
Shy bladder occurs when a donor cannot provide the required volume of urine (at least 30 mL) within the allotted collection time.
Under DOT regulations, what is the minimum volume of urine required for a primary specimen in a federal workplace drug test?