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Instrument Processing Flashcards

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  1. Which enzyme is most commonly used in enzymatic detergents to break down protein-based bioburden on surgical instruments?

    Answer: Protease

    Protease enzymes specifically break down proteins, which constitute the majority of blood and tissue soils on surgical instruments.

  2. During mechanical decontamination in a washer-disinfector, what temperature range is typically used in the thermal disinfection cycle to achieve pasteurization?

    Answer: 71–80°C (160–176°F)

    Thermal disinfection in washer-disinfectors typically uses 71–80°C for a specified time to achieve pasteurization-level disinfection.

  3. A hinged instrument is opened before sterilization primarily to:

    Answer: Allow steam or sterilant contact on all surfaces including box locks

    Opening hinged instruments ensures sterilant reaches the box lock area, which is a critical contact point that cannot be penetrated when the instrument is closed.

  4. Which of the following best describes 'critical' items in the Spaulding Classification System?

    Answer: Items that enter sterile tissue or the vascular system and require sterilization

    Critical items enter sterile body tissue or the vascular system and pose the highest infection risk, requiring sterilization before use.

  5. What is the purpose of a biological indicator (BI) in sterilization monitoring?

    Answer: To demonstrate that viable microorganisms were killed during the sterilization process

    Biological indicators contain highly resistant spores; their destruction after processing confirms the sterilization process was effective at killing microorganisms.

  6. When using ultrasonic cleaners, cavitation refers to:

    Answer: The formation and collapse of microscopic bubbles that dislodge soil

    Cavitation is the rapid formation and implosion of tiny bubbles created by high-frequency sound waves, which physically dislodge soil from instrument surfaces.

  7. Which sterilization method is most appropriate for moisture-sensitive, heat-sensitive instruments such as flexible endoscopes?

    Answer: Ethylene oxide (EO) gas sterilization

    Ethylene oxide sterilization uses low temperatures and is appropriate for heat- and moisture-sensitive instruments that cannot withstand steam.